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The world’s first plastic culture bag technology demonstration experiment performed at the ISS’s Japanese Experiment Module “Kibo”

  • Research and Technology

December 22, 2021

Japan Aerospace Exploration Agency
Takenaka Corporation
Kirin Holdings Company, Limited
Chiba University
Tokyo University of Science

TOKYO, Wednesday December 22, 2021 – The Japan Aerospace Exploration Agency (JAXA), Takenaka Corporation, Kirin Holdings Company, Limited (Kirin Holdings), Chiba University, and Tokyo University of Science, aiming at food production during long-term stays in space for future lunar and other exploration missions, carried out a demonstration experiment of the plastic culture bagtechnology at the Japanese Experiment Module “Kibo” on the International Space Station (ISS). This was a first of its kind in the world.

  • Lettuce cultivated in a sealed bag
    (Left: before harvesting; Right: before collecting on the ground)

  • International Space Station (ISS)

  • Location of experimental equipment installed in “Kibo”

Background of Experiment

As the space development sector is seeing momentum gathering for expanding the sphere of human activities to near-Earth objects, such as the moon and Mars, JAXA is promoting technology development to serve this purpose. One of such research projects aims to set up farms on the moon and produce food to enable long-term stays with reduced supply from the Earth. In the future plan for farming on the lunar surface, disease and pest control and emergency food supply will be the key to sustained stable production. Also, cultivation systems will need to be downsized to be transported appropriately from the Earth.

In this context, JAXA, Takenaka Corporation, Kirin Holdings Co., Ltd., Chiba University, and Tokyo University of Science teamed up in a framework for the open call for joint research proposals of the JAXA Space Exploration Innovation Hub, and started in 2017 to develop the plastic culture bag technology with a view to application to space activities (*1). The technology is to grow plants in a small closed bag, which can prevent bacterial contamination and odor leakage. The cultivation system is housed in compact simple equipment, which enables easy maintenance, energy saving, and flexible control of production volume (Fig. 1). The recent demonstration was planned to confirm achievements from the team’s past efforts. The experiment was performed in Kibo using lettuce to assess the effectiveness of the cultivation method in a micro-gravity, closed environment, and advantages over hydroponics and soil cultivation.
*1. The related joint research agreement was concluded in 2017 for the “research on a farm system free from pathogens and insects and capable of backing up in emergency,” which was adopted for JAXA’s “Open Innovation Hub for Expanding Humanosphere and the Domain of Human Activity through Solar System Frontier Development (Open Innovation Hub),” selected by the Japan Science and Technology Agency to support with starting up innovation hub projects.

Outline of Experiment

The experiment was conducted for 48 days from August 27 to October 13, 2021. During the course of the period, cultivation was promoted by ensuring the supply of a culture solution and exchange of air. True leaves of lettuce were confirmed on September 10, and subsequently the vegetable continued to grow steadily to be successfully harvested.
The cultivation device, measuring 44 (w) x 35 (d) x 20 (h) cm and weighing 5 kg, was designed to be compact to diminish the load on transportation. The system can grow up to three bags of plants, and includes functions to sterilize drinking water in the ISS to create and supply a culture solution and automatically photograph the growth status on a periodical basis.

  • Demonstration cultivation device

Following the experiment, grown lettuce, culture medium, and growth records will be collected to evaluate the advantages and applicability in space of this cultivation method. Also, investigation will be conducted for hygienic safety of the harvests while analyzing the culture medium to explore the possibility of reuse for the ISS’ environmental control and life support systems (*2).
*2. The environmental control and life support system (ECLSS) installed in the ISS. Equipped with physicochemical mechanisms to supply clean air and water to the crew and experiment animals.

Expected future application

Major expected future applications of the plastic culture bag technology include mass production of leafy vegetables and virus-free growth of seedlings, which will support long-duration manned planetary exploration missions and large-scale cultivation in spacecraft in orbit and/or accommodation on the moon (Fig. 2). The joint research project will continue to further develop the technology to be able to contribute to sustainable space activities.

  • Fig. 1 Cultivation in plastic culture bags on the ground

  • Fig. 2 Farming on the moon (image model)

About Kirin Holdings

Kirin Holdings Company, Limited is an international company that operates in the Food & Beverages domain (Food & Beverages businesses), Pharmaceuticals domain (Pharmaceuticals businesses), and Health Science domain (Health Science business), both in Japan and across the globe.
Kirin Holdings can trace its roots to Japan Brewery which was established in 1885. Japan Brewery became Kirin Brewery in 1907. Since then, the company expanded its business with fermentation and biotechnology as its core technologies, and entered the pharmaceutical business in the 1980s, all of which continue to be global growth centers. In 2007, Kirin Holdings was established as a pure holding company and is currently focusing on boosting its Health Science domain.
Under the Kirin Group Vision 2027 (KV 2027), a long-term management plan launched in 2019, the Kirin Group aims to become A global leader in CSV*, creating value across our world of Food & Beverages to Pharmaceuticals. Going forward, the Kirin Group will continue to leverage its strengths to create both social and economic value through its businesses, with the aim of achieving sustainable growth in corporate value.
* Creating Shared Value: combined added value for consumers as well as for society at large.


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